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(Solved by Expert Tutors) You set up the following bacterial conjugation experiment:? Hfr??tetR,?


mated to F-??tetS,?lac-,?leu-,?strR?(where?tetR?indicates resistance to tetracycline;?lac-?indicates inability to utilize lactose as a carbon source;?leu-?indicates leucine auxotrophy; and?strRindicates resistance to streptomycin).? At various times, the mating pairs are separated by a quick pulse in a blender.? The cells are then plated on the three types of agar media described below:??

?

media 1 = Rich media + glucose + tetracycline + streptomycin

?

media 2 = Minimal media + lactose + leucine + streptomycin

?

media 3 = Minimal media + glucose + streptomycin


The following table shows the number of colonies on each type of media for samples taken at various times after the strains were mixed:

Table attached file.

The following maps represent linearized images of the entire Hfr chromosome with the arrow in the center indicating oriT (origin of transfer).? The point of the arrow represents the first nucleotides that enter the recipient during conjugation.? Which map is most consistent with this data??

attached abcd

 


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Apr 19, 2020

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